Visible Fluorescence Detection of Type III Protein Secretion from Bacterial Pathogens

详细信息    查看全文

• 作者：Jacob S. Yount ; Lun K. Tsou ; Paul D. Dossa ; Amy L. Kullas ; Adrianus W. M. van der Velden ; Howard C. Hang
• 刊名：Journal of the American Chemical Society
• 出版年：2010
• 出版时间：June 23, 2010
• 年：2010
• 卷：132
• 期：24
• 页码：8244-8245
• 全文大小：157K
• 年卷期：v.132,no.24(June 23, 2010)
• ISSN：1520-5126

文摘

Type III protein secretion is essential for many Gram-negative bacterial infections of host cells and an attractive target for new antibacterial drugs. Here, we describe a bacterial protein effector-carboxypeptidase G2 (CPG2) reporter system for fluorescence and visible detection of type III protein secretion in Salmonella typhimurium. This system provides a general method for measuring protein expression and secretion as well as a high-throughput and quantitative assay for analyzing type III protein secretion inhibitors.