Engineering Transaldolase in Pichia stipitis to Improve Bioethanol Production

详细信息    查看全文

• 作者：Shan-He Chen ; Der-Ren Hwang ; Gan-Hong Chen ; Ning-Shian Hsu ; Ying-Ta Wu ; Tsung-Lin Li ; Chi-Huey Wong
• 刊名：ACS Chemical Biology
• 出版年：2012
• 出版时间：March 16, 2012
• 年：2012
• 卷：7
• 期：3
• 页码：481-486
• 全文大小：367K
• 年卷期：v.7,no.3(March 16, 2012)
• ISSN：1554-8937

文摘

In our effort to improve the efficiency and yield of xylose-to-ethanol bioconversion in Pichia stipitis, the transaldolase (TAL) in the pentose phosphate pathway was identified as a rate-limiting enzyme for improvement. A mutant containing the Q263R change was first obtained by directed evolution with 5-fold increase of activity, which was then incorporated into P. stipitesvia the pYDS vector to produce a genetically stable strain for fermentation on xylose. In comparison with the parental strain, TAL-Q263R(+) increases ethanol prodcution by 36% and 100% as measured by volumetric production rate and specific production rate, respectively. Thus improving the transaldolase activity in P. stipitis can significantly increase the rate and yield of xylose conversion to ethanol.