Spatial Confinement Induced Enzyme Stability for Bioelectronic Applications

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• 作者：Gang Wang ; Siu-Tung Yau
• 刊名：Journal of Physical Chemistry C
• 出版年：2007
• 出版时间：August 16, 2007
• 年：2007
• 卷：111
• 期：32
• 页码：11921 - 11926
• 全文大小：321K
• 年卷期：v.111,no.32(August 16, 2007)
• ISSN：1932-7455

文摘

The stability of glucose oxidase (GOx) immobilized on a silicon electrode can be enhanced using spatialconfinement as described in this paper. We show that when GOx is assembled in semiopen spatially confinedstructures created on the unmodified surface of a silicon electrode, the enzyme's activity is preserved underdestabilizing conditions. GOx-immobilized silicon electrodes were treated with guanidinium chloride andhigh temperature. Cyclic voltammetry measurement of the treated electrodes showed the proper redoxcharacteristics of GOx and indicated the GOx-catalyzed electrooxidation of glucose. When GOx wasimmobilized on flat silicon electrodes, voltammetric measurement showed null result presumably caused bydenaturation of the enzyme. The effect of spatial confinement on enzyme stability is also revealed by analyzingthe characteristic rate constants and the kinetic parameter for the complete catalytic process of glucose.