Application of Stable Carbon Isotope Analysis to the Detection of Testosterone Administration to Cattle
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- 作者：Moritz Hebestreit ; Ulrich Flenker ; Corinne Buisson ; Francois Andre ; Bruno Le Bizec ; Hildburg Fry ; Melanie Lang ; Angelika Preiss Weigert ; Katharina Heinrich ; Simon Hird ; and Wilhelm Schä ; nzer
- 刊名：Journal of Agricultural and Food Chemistry
- 出版年：2006
- 出版时间：April 19, 2006
- 年：2006
- 卷：54
- 期：8
- 页码：2850 - 2858
- 全文大小：170K
- 年卷期：v.54,no.8(April 19, 2006)
- ISSN：1520-5118
文摘
The use of anabolic substances is prohibited in food-producing animals throughout the EuropeanUnion. No method is available to reliably detect the misuse of natural hormones in cattle. A methodwas developed to detect the abuse of testosterone in cattle fattening. Synthesized testosterone israther depleted in the 13C/12C ratio. Hence, the method is based on gas chromatography/combustion/isotope ratio mass spectrometry (GC/C/IRMS) analysis of urine. To select testosterone metabolitesand endogenous reference compounds (ERC), the concentration of urinary steroids of cattle wasinvestigated. Dehydroepiandrosterone and androst-5ene-3
,17
-diol were chosen as ERCs to showendogenous 13C/12C ratios. Etiocholanolone and 5-androstane-3,17-diol were chosen as the mostimportant testosterone metabolites. Other metabolites known from literature like epitestosterone wereless promising. In principle, GC/C/IRMS is a nonspecific method because finally carbon dioxide isanalyzed. Therefore, a dedicated cleanup procedure for the selected steroids was developed. Bymeans of proposed confidence intervals in the isotopic composition of ERCs and metabolites, theadministration of testosterone to cattle could be detected reliably. Differences of up to 11” on the
-scale between ERC and testosterone metabolites were found after testosterone administration,whereas endogenous differences did not exceed 2”.Keywords: IRMS; testosterone; steroids; stable carbon isotopes; natural hormones; bovine; cattle;urine
,17-diol were chosen as ERCs to showendogenous 13C/12C ratios. Etiocholanolone and 5-androstane-3,17-diol were chosen as the mostimportant testosterone metabolites. Other metabolites known from literature like epitestosterone wereless promising. In principle, GC/C/IRMS is a nonspecific method because finally carbon dioxide isanalyzed. Therefore, a dedicated cleanup procedure for the selected steroids was developed. Bymeans of proposed confidence intervals in the isotopic composition of ERCs and metabolites, theadministration of testosterone to cattle could be detected reliably. Differences of up to 11” on the-scale between ERC and testosterone metabolites were found after testosterone administration,whereas endogenous differences did not exceed 2”.Keywords: IRMS; testosterone; steroids; stable carbon isotopes; natural hormones; bovine; cattle;urine