Quantitative Imaging of Gold and Silver Nanoparticles in Single Eukaryotic Cells by Laser Ablation ICP-MS

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• 作者：Daniela Drescher ; Charlotte Giesen ; Heike Traub ; Ulrich Panne ; Janina Kneipp ; Norbert Jakubowski
• 刊名：Analytical Chemistry
• 出版年：2012
• 出版时间：November 20, 2012
• 年：2012
• 卷：84
• 期：22
• 页码：9684-9688
• 全文大小：307K
• 年卷期：v.84,no.22(November 20, 2012)
• ISSN：1520-6882

文摘

Laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) was utilized for spatially resolved bioimaging of the distribution of silver and gold nanoparticles in individual fibroblast cells upon different incubation experiments. High spatial resolution was achieved by optimization of scan speed, ablation frequency, and laser energy. Nanoparticles are visualized with respect to cellular substructures and are found to accumulate in the perinuclear region with increasing incubation time. On the basis of matrix-matched calibration, we developed a method for quantification of the number of metal nanoparticles at the single-cell level. The results provide insight into nanoparticle/cell interactions and have implications for the development of analytical methods in tissue diagnostics and therapeutics.