Silane-Modified Magnetic Beads: Application to Immunoglobulin G Separation
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- 作者：Nevra Ö ; ztü ; rk ; M. Emin Gü ; nay ; Sinan Akgö ; l ; Adil Denizli
- 刊名：Biotechnology Progress
- 出版年：2007
- 出版时间：October 2007
- 年：2007
- 卷：23
- 期：5
- 页码：1149 - 1156
- 全文大小：437K
- 年卷期：v.23,no.5(October 2007)
- ISSN：1520-6033
文摘
The magnetic poly(2-hydroxyethyl methacrylate ethylene glycol dimethacrylate) [m-poly(HEMA-EGDMA)] beads (150-250-m diameter in spherical form) were prepared by a radical suspensionpolymerization technique. The pseudo-specific ligand, reactive imidazole containing 3-(2-imidazoline-1-yl)propyl (triethoxysilane) (IMEO) was selected as a silanization agent. IMEOwas covalently immobilized onto the magnetic beads. IMEO-immobilized m-poly(HEMA-EGDMA) beads were used for the affinity adsorption of immunoglobulin-G (IgG) from aqueoussolutions and human plasma. To evaluate the degree of IMEO attachment, the m-poly(HEMA-EGDMA) beads were subjected to Si analysis by using flame atomizer atomic absorptionspectrometer, and it was estimated as 36.6 mg IMEO/g of polymer. The nonspecific IgGadsorption onto the plain m-poly(HEMA-EGDMA) beads was very low (about 0.4 mg/g). Higheradsorption values (up to 55 mg/g) were obtained when the m-poly(HEMA-EGDMA)/IMEObeads were used from both aqueous solutions and human plasma. The maximum IgG adsorptionon the m-poly(HEMA-EGDMA)-IMEO beads was observed at pH 7.0. The IgG molecules couldbe repeatedly adsorbed and desorbed with m-poly(HEMA-EGDMA)-IMEO beads withoutnoticeable loss in the IgG adsorption capacity. The adsorption capacity from human plasma inmagnetically stabilized fluidized bed decreased drastically from 78.9 to 19.6 mg/g with theincrease of the flow rate from 0.2 to 3.5 mL/min.