A Novel Parallel Shaken Bioreactor System for Continuous Operation
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- 作者：Ali Akgü ; n ; Bernd Maier ; Diana Preis ; Birthe Roth ; Renata Klingelhö ; fer ; and Jochen Bü ; chs
- 刊名：Biotechnology Progress
- 出版年：2004
- 出版时间：December 2004
- 年：2004
- 卷：20
- 期：6
- 页码：1718 - 1724
- 全文大小：235K
- 年卷期：v.20,no.6(December 2004)
- ISSN：1520-6033
文摘
A novel continuous bioreactor system was developed as a shaken culture vessel forthe investigation of the growth kinetics and product formation of microorganisms inmilliscale. The novel bioreactor system mainly consists of a specially designed 250-mL shake flask with two inlets, one for gas supply and one for medium supply, andone combined outlet on the side of flask for exhaust gas and culture liquid. As a resultof the circulating motion of the fermentation broth in the shake flask, the maximumliquid height reaches the edge of the outlet and the fermentation broth is acceleratedinto the outlet by centrifugal force. Additionally, the excess fermentation broth leavingthe culture vessel is continuously driven by the exhaust gas. Because of the smallscale and the simple handling it is possible to operate many of these shaken bioreactorvessels simultaneously. By using parallel vessels operated at different dilution rateson the same shaker, the data for a complete biomass over dilution rate (X-D) diagramof a biological culture can be evaluated in an efficient manner, thus saving money,materials, and time. Continuous fermentations of the yeast Saccharomyces cerevisiaeH1022 (ATCC 32167) in the shaken bioreactor system and in a conventional stirredtank fermentor showed very similar results.