文摘
The adsorption isotherms of oleuropein and rutin were evaluated at different temperatures, pH values,and solid/liquid ratios. The experimental data of adsorption isotherms were well fitted to a Langmuirmodel. The maximum adsorption capacities were determined as 108 mg of oleuropein/g of silk fibroinand 21 mg of rutin/g of silk fibroin. After adsorption of oleuropein and rutin, the antioxidant capacityof silk fibroin increased from 1.93 to 3.61 mmol of TEAC/g. Silk fibroin also gained antimicrobialactivity against Staphylococcus aureus and Klebsiella pneumoniae after adsorption of olive leafantioxidants. In a desorption process, 81% of rutin and 85% of oleuropein were removed from theadsorbent surface in 70% aqueous ethanol solution. Consequently, silk fibroin was found to be apromising biomaterial for the production of functional food or dietary supplements and for thepurification of oleuropein and rutin from olive leaf extracts.