Skin Dendritic Cell Targeting via Microneedle Arrays Laden with Antigen-Encapsulated Poly-d,l-lactide-co-Glycolide Nanoparti
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- 作者：Marija Zaric ; Oksana Lyubomska ; Olivier Touzelet ; Candice Poux ; Sharifah Al-Zahrani ; Francois Fay ; Leah Wallace ; Dorothea Terhorst ; Bernard Malissen ; Sandrine Henri ; Ultan F. Power ; Christopher J. Scott ; Ryan F. Donnelly ; Adrien Kissenpfennig
- 刊名：ACS Nano
- 出版年：2013
- 出版时间：March 26, 2013
- 年：2013
- 卷：7
- 期：3
- 页码：2042-2055
- 全文大小：899K
- 年卷期：v.7,no.3(March 26, 2013)
- ISSN：1936-086X
文摘
The efficacious delivery of antigens to antigen-presenting cells (APCs), in particular, to dendritic cells (DCs), and their subsequent activation remains a significant challenge in the development of effective vaccines. This study highlights the potential of dissolving microneedle (MN) arrays laden with nanoencapsulated antigen to increase vaccine immunogenicity by targeting antigen specifically to contiguous DC networks within the skin. Following in situ uptake, skin-resident DCs were able to deliver antigen-encapsulated poly-d,l-lactide-co-glycolide (PGLA) nanoparticles to cutaneous draining lymph nodes where they subsequently induced significant expansion of antigen-specific T cells. Moreover, we show that antigen-encapsulated nanoparticle vaccination via microneedles generated robust antigen-specific cellular immune responses in mice. This approach provided complete protection in vivo against both the development of antigen-expressing B16 melanoma tumors and a murine model of para-influenza, through the activation of antigen-specific cytotoxic CD8+ T cells that resulted in efficient clearance of tumors and virus, respectively. In addition, we show promising findings that nanoencapsulation facilitates antigen retention into skin layers and provides antigen stability in microneedles. Therefore, the use of biodegradable polymeric nanoparticles for selective targeting of antigen to skin DC subsets through dissolvable MNs provides a promising technology for improved vaccination efficacy, compliance, and coverage.