Comparative Proteomic Analysis of Two Varieties of Genetically Modified (GM) Embrapa 5.1 Common Bean (Phaseolus vulgaris L.) and Their Non-GM Counterparts

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• 作者：Geisi M. Balsamo ; Pedro A. Valentim-Neto ; Carla S. Mello ; Ana C. M. Arisi
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2015
• 出版时间：December 9, 2015
• 年：2015
• 卷：63
• 期：48
• 页码：10569-10577
• 全文大小：421K
• ISSN：1520-5118

文摘

The genetically modified (GM) common bean event Embrapa 5.1 was commercially approved in Brazil in 2011; it is resistant to golden mosaic virus infection. In the present work grain proteome profiles of two Embrapa 5.1 common bean varieties, P茅rola and Pontal, and their non-GM counterparts were compared by two-dimensional gel electrophoresis (2-DE) followed by mass spectrometry (MS). Analyses detected 23 spots differentially accumulated between GM P茅rola and non-GM P茅rola and 21 spots between GM Pontal and non-GM Pontal, although they were not the same proteins in P茅rola and Pontal varieties, indicating that the variability observed may not be due to the genetic transformation. Among them, eight proteins were identified in P茅rola varieties, and four proteins were identified in Pontal. Moreover, we applied principal component analysis (PCA) on 2-DE data, and variation between varieties was explained in the first two principal components. This work provides a first 2-DE-MS/MS-based analysis of Embrapa 5.1 common bean grains.