Chemical Composition of the Bark of Tetrapterys mucronata and Identification of Acetylcholinesterase Inhibitory Constituents

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• 作者：Marcos Mar莽al Ferreira Queiroz ; Emerson Ferreira Queiroz ; Maria Luiza Zeraik ; Samad Nejad Ebrahimi ; Laurence Marcourt ; Muriel Cuendet ; Ian Castro-Gamboa ; Matthias Hamburger ; Vanderlan da Silva Bolzani ; Jean-Luc Wolfender
• 刊名：Journal of Natural Products
• 出版年：2014
• 出版时间：March 28, 2014
• 年：2014
• 卷：77
• 期：3
• 页码：650-656
• 全文大小：352K
• 年卷期：v.77,no.3(March 28, 2014)
• ISSN：1520-6025

文摘

The secondary metabolite content of Tetrapterys mucronata, a poorly studied plant that is used occasionally in Brazil for the preparation of a psychotropic plant decoction called 鈥淎yahuasca鈥? was determined to establish its chemical composition and to search for acetylcholinesterase (AChE) inhibitors. The ethanolic extract of the bark of T. mucronata exhibited in vitro AChE inhibition in a TLC bioautography assay. To localize the active compounds, biological profiling for AChE inhibition was performed using at-line HPLC-microfractionation in 96-well plates and subsequent AChE inhibition bioautography. The analytical HPLC-PDA conditions were transferred geometrically to a preparative medium-pressure liquid chromatography column using chromatographic calculations for the efficient isolation of the active compounds at the milligram scale. Twenty-two compounds were isolated, of which six are new natural products. The structures of the new compounds (9, 10, 16鈥?b>18, and 20) were elucidated by spectroscopic data interpretation. Compounds 1, 5, 6, 9, and 10 inhibited AChE with IC<sub>50sub> values below 15 渭M.