纬-Oryzanol Reduces Adhesion Molecule Expression in Vascular Endothelial Cells via Suppression of Nuclear Factor-魏B Activation
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- 作者：Satoshi Sakai ; Takahisa Murata ; Yoshiki Tsubosaka ; Hideki Ushio ; Masatoshi ; Hori ; Hiroshi Ozaki
- 刊名：Journal of Agricultural and Food Chemistry
- 出版年：2012
- 出版时间：April 4, 2012
- 年：2012
- 卷：60
- 期：13
- 页码：3367-3372
- 全文大小：314K
- 年卷期：v.60,no.13(April 4, 2012)
- ISSN：1520-5118
文摘
纬-Oryzanol (纬-ORZ) is a mixture of phytosteryl ferulates purified from rice bran oil. In this study, we examined whether 纬-ORZ represents a suppressive effect on the lipopolysaccharide (LPS)-induced adhesion molecule expression on vascular endothelium. Treatment with LPS elevated the mRNA expression of vascular cell adhesion molecule-1 (VCAM-1), intercellular adhesion molecule-1 (ICAM-1), and E-selectin in bovine aortic endothelial cells (BAECs). Pretreatment with 纬-ORZ dose-dependently decreased the LPS-mediated expression of these genes. Western blotting also revealed that pretreatment with 纬-ORZ dose-dependently inhibited LPS-induced VCAM-1 expression in human umbilical vein endothelial cells. Consistently, pretreatment with 纬-ORZ dose-dependently reduced LPS-induced U937 monocyte adhesion to BAECs. In immunofluorescence, LPS caused nuclear factor-魏B (NF-魏B) nuclear translocation in 40% of BAECs, which indicates NF-魏B activation. Pretreatment with 纬-ORZ, as well as its components (cycloartenyl ferulate, ferulic acid, or cycloartenol), dose-dependently inhibited LPS-mediated NF-魏B activation. Collectively, our results suggested that 纬-ORZ reduced LPS-mediated adhesion molecule expression through NF-魏B inhibition in vascular endothelium.