Relative Quantification of Stable Isotope Labeled Peptides Using a Linear Ion Trap-Orbitrap Hybrid Mass Spectrometer

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• 作者：John D. Venable ; James Wohlschlegel ; Daniel B. McClatchy ; Sung Kyu Park ; John R. Yates ; III
• 刊名：Analytical Chemistry
• 出版年：2007
• 出版时间：April 15, 2007
• 年：2007
• 卷：79
• 期：8
• 页码：3056 - 3064
• 全文大小：470K
• 年卷期：v.79,no.8(April 15, 2007)
• ISSN：1520-6882

文摘

The quantitative analysis of complex biological sampleshas emerged as a key research area in the field ofproteomics. Although quantitative proteomic experimentsremain challenging, these strategies have been greatlyfacilitated by the development of newer high-performancemass spectrometers. In this work, we have evaluated theuse of the LTQ-Orbitrap, a hybrid mass spectrometer inwhich a linear ion trap is coupled to an Orbitrap massanalyzer, for quantitative analyses. By analyzing a rangeof yeast protein standards, we found that the high massaccuracy, high resolution, large ion capacity, and largedynamic range of the LTQ-Orbitrap led to as much as a4-5-fold improvement in the number and quality of thepeptide ratio measurements compared to similar analysesdone on the LTQ. We also successfully quantified proteinexpression differences that occur in metabolically labeledrat synapses during brain development to further demonstrate the suitability of the LTQ-Orbitrap for the comparative analysis of complex tissue samples.