Polypeptide Semicarbazide Glass Slide Microarrays: Characterization and Comparison with Amine Slides in Serodetection Studies
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- 作者：Xavier Duburcq ; Christophe Olivier ; Ré ; mi Desmet ; Matej Halasa ; Olivier Carion ; Bruno Grandidier ; Thomas Heim ; Didier Stié ; venard ; Claude Auriault ; and Oleg Melnyk
- 刊名：Bioconjugate Chemistry
- 出版年：2004
- 出版时间：March 2004
- 年：2004
- 卷：15
- 期：2
- 页码：317 - 325
- 全文大小：282K
- 年卷期：v.15,no.2(March 2004)
- ISSN：1520-4812
文摘
We have described in the accompanying article the preparation of peptide-protein semicarbazidemicroarrays and their use for the simultaneous serodetection of antibodies directed against differentpathogens. Here, we present a comparative study between semicarbazide and amine glass slides inan immunofluorescent serodetection assay using HIV (Gp120, Gp41), HCV (mix-HCV, core, NS3, andNS4), and HBV (HBs) recombinant antigens. Amine and semicarbazide surfaces displayed the samesensitivity for antibodies detection just after printing. However, the reactivity of protein antigenschanged rapidly upon aging on amine slides but not on semicarbazide slides. Peptide or proteinsemicarbazide microarrays were found to be remarkably stable for months. Additional data concerningthe characterization of the semicarbazide surface (homogeneity of the slides, chemical stability, contactangle measurements, atomic force microscopy studies, reproducibility of serodetection results) arealso presented and discussed.