Direct Characterization of Native Chemical Ligation of Peptides on Silicon Nanowires

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• 作者：Nabil Dendane ; Oleg Melnyk ; Tao Xu ; Bruno Grandidier ; Rabah Boukherroub ; Didier Sti茅venard ; Yannick Coffinier
• 刊名：Langmuir
• 出版年：2012
• 出版时间：September 18, 2012
• 年：2012
• 卷：28
• 期：37
• 页码：13336-13344
• 全文大小：402K
• 年卷期：v.28,no.37(September 18, 2012)
• ISSN：1520-5827

文摘

We describe the site-specific and chemoselective immobilization of peptides on hydrogen-terminated silicon nanowires (SiNWs) using native chemical ligation (NCL) (i.e., the reaction of a thioester group with a cysteine moiety to give a stable amide bond). The SiNWs investigated in this work were grown via a vapor鈥搇iquid鈥搒olid mechanism and functionalized with a thioester moiety. The immobilization of the peptides on the SiNWs was demonstrated by synthesizing peptides with an N-terminal cysteine residue and labeled with tetramethylrhodamine or trifluoromethyl groups that were detected by fluorescence and X-ray photoelectron spectroscopy, respectively. The peptides labeled with tetramethylrhodamine or trifluoromethyl groups for fluorescence or X-ray photoelectron spectroscopy (XPS) detection studies were synthesized with an N-terminal cysteine residue. N-Terminal seryl peptides and carboxy-terminated SiNWs were used as controls to demonstrate the chemoselectivity of the peptide immobilization.