Impact of Phenolic Substrate and Growth Temperature on the Arthrobacter chlorophenolicus Proteome

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• 作者：Maria Unell^† ; ; Paul E. Abraham^‡ ; ; Manesh Shah^§ ; ; Bing Zhang ; Christian Rckert ; Nathan C. VerBerkmoes^‡ ; ; J
• 刊名：Journal of Proteome Research
• 出版年：2009
• 出版时间：April 3, 2009
• 年：2009
• 卷：8
• 期：4
• 页码：1953-1964
• 全文大小：753K
• 年卷期：v.8,no.4(April 3, 2009)
• ISSN：1535-3907

文摘

We compared the Arthrobacter chlorophenolicus proteome during growth on 4-chlorophenol, 4-nitrophenol, or phenol at 5 and 28 °C, both for the wild-type and a mutant strain with mass spectrometry based proteomics. A label-free workflow employing spectral counting identified 3749 proteins across all growth conditions, representing over 70% of the predicted genome and 739 of these proteins form the core proteome. Statistically significant differences were found in the proteomes of cells grown under different conditions including differentiation of hundreds of unknown proteins. The 4-chlorophenol-degradation pathway was confirmed, but not that for phenol.