Analysis of Native Biological Surfaces Using a 100 kV Massive Gold Cluster Source

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• 作者：Francisco A. Fernandez-Lima ; Jeremy Post ; John D. DeBord ; Michael J. Eller ; Stanislav V. Verkhoturov ; Serge Della-Negra ; Amina S. Woods ; Emile A. Schweikert
• 刊名：Analytical Chemistry
• 出版年：2011
• 出版时间：November 15, 2011
• 年：2011
• 卷：83
• 期：22
• 页码：8448-8453
• 全文大小：917K
• 年卷期：v.83,no.22(November 15, 2011)
• ISSN：1520-6882

文摘

In the present work, the advantages of a new, 100 kV platform equipped with a massive gold cluster source for the analysis of native biological surfaces are shown. Inspection of the molecular ion emission as a function of projectile size demonstrates a secondary ion yield increase of 100脳 for 520 keV Au<sub>400sub><sup>4+sup> as compared to 130 keV Au<sub>3sub><sup>1+sup> and 43 keV C<sub>60sub>. In particular, yields of tens of percent of molecular ions per projectile impact for the most abundant components can be observed with the 520 keV Au<sub>400sub><sup>4+sup> probe. A comparison between 520 keV Au<sub>400sub><sup>4+sup> time-of-flight-secondary ion mass spectrometry (TOF-SIMS) and matrix assisted laser desorption ionization-mass spectrometry (MALDI-MS) data showed a similar pattern and similar relative intensities of lipid components across a rat brain sagittal section. The abundant secondary ion yield of analyte-specific ions makes 520 keV Au<sub>400sub><sup>4+sup> projectiles an attractive probe for submicrometer molecular mapping of native surfaces.