Isoflavonoid Composition of a Callus Culture of the Relict Tree Maackia amurensis Rupr. et Maxim

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• 作者：Sergey A. Fedoreyev ; Victor P. Bulgakov ; Olga V. Grishchenko ; Marina V. Veselova ; Olga E. Krivoschekova ; Nadezda I. Kulesh ; Vladimir A. Denisenko ; Galina K. Tchernoded ; Yury N. Zhuravlev
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2008
• 出版时间：August 27, 2008
• 年：2008
• 卷：56
• 期：16
• 页码：7023-7031
• 全文大小：220K
• 年卷期：v.56,no.16(August 27, 2008)
• ISSN：1520-5118

文摘

Isoflavonoids, an interesting and restricted group of secondary metabolites of legumes, exhibit estrogenic, antiangiogenic, and anticancer activities and are now popular as dietary supplements. Plant cell cultures that possess an increased ability to synthesize these metabolites were examined. During the investigation, cell cultures of the Far Eastern relict tree amed-content content-type="genus-species" type="simple">Maackia amurensisamed-content> (Leguminosae) were established. A selection of seed-derived cell aggregates yielded the callus line designated A-18. This culture produces 20 isoflavonoids, namely, the isoflavones genistein, daidzein, formononetin, calycosin, derrone, and pseudobaptigenin and their glycosylated conjugates genistin, 6′′-O-malonylgenistin, ononin, 6′′-O-malonylononin, daidzin, 3′-methoxydaidzin, 4′-O-β-pan class="smallcaps">dpan>-glucopyranosyldaidzin, 4′-O-β-pan class="smallcaps">dpan>-glucopyranosylgenistin, and 7-O-β-pan class="smallcaps">dpan>-glucopyranosylcalycosin; the pterocarpans maackiain and medicarpin and their glycosylated conjugates 6′-O-malonyl-3-O-β-pan class="smallcaps">dpan>-glucopyranosylmaackiain and 6′-O-malonyl-3-O-β-pan class="smallcaps">dpan>-glucopyranosylmedicarpin; and the new pterocarpan glucoside 6′-O-malonyl-3-O-β-pan class="smallcaps">dpan>-glucopyranosyl-6,6a-dehydromaackiain. These isoflavonoids, possessing a hepatoprotective activity, were stably produced by the A-18 calli for prolonged periods of observation.