Nanotopography Influences Adhesion, Spreading, and Self-Renewal of Human Embryonic Stem Cells
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- 作者：Weiqiang Chen ; Luis G. Villa-Diaz ; Yubing Sun ; Shinuo Weng ; Jin Koo Kim ; Raymond H. W. Lam ; Lin Han ; Rong Fan ; Paul H. Krebsbach ; Jianping Fu
- 刊名：ACS Nano
- 出版年：2012
- 出版时间：May 22, 2012
- 年：2012
- 卷：6
- 期：5
- 页码：4094-4103
- 全文大小：572K
- 年卷期：v.6,no.5(May 22, 2012)
- ISSN：1936-086X
文摘
Human embryonic stem cells (hESCs) have great potentials for future cell-based therapeutics. However, their mechanosensitivity to biophysical signals from the cellular microenvironment is not well characterized. Here we introduced an effective microfabrication strategy for accurate control and patterning of nanoroughness on glass surfaces. Our results demonstrated that nanotopography could provide a potent regulatory signal over different hESC behaviors, including cell morphology, adhesion, proliferation, clonal expansion, and self-renewal. Our results indicated that topological sensing of hESCs might include feedback regulation involving mechanosensory integrin-mediated cell鈥搈atrix adhesion, myosin II, and E-cadherin. Our results also demonstrated that cellular responses to nanotopography were cell-type specific, and as such, we could generate a spatially segregated coculture system for hESCs and NIH/3T3 fibroblasts using patterned nanorough glass surfaces.