The Membrane-Active Regions of the Hepatitis C Virus E1 and E2 Envelope Glycoproteins

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• 作者：Ana J. Pé ; rez-Berná ; Miguel R. Moreno ; Jaime Guillé ; n ; Angela Bernabeu ; and José ; Villala&iacute ; n
• 刊名：Biochemistry
• 出版年：2006
• 出版时间：March 21, 2006
• 年：2006
• 卷：45
• 期：11
• 页码：3755 - 3768
• 全文大小：1109K
• 年卷期：v.45,no.11(March 21, 2006)
• ISSN：1520-4995

文摘

We have identified the membrane-active regions of the full sequences of the HCV E1 and E2envelope glycoproteins by performing an exhaustive study of membrane leakage, hemifusion, and fusioninduced by 18-mer peptide libraries on model membranes having different phospholipid compositions.The data and their comparison have led us to identify different E1 and E2 membrane-active segmentswhich might be implicated in viral membrane fusion, membrane interaction, and/or protein-protein binding.Moreover, it has permitted us to suggest that the fusion peptide might be located in the E1 glycoproteinand, more specifically, the segment comprised by amino acid residues 265-296. The identification ofthese membrane-active segments from the E1 and E2 envelope glycoproteins, as well as theirmembranotropic propensity, supports their direct role in HCV-mediated membrane fusion, sustains thenotion that different segments provide the driving force for the merging of the viral and target cellmembranes, and defines those segments as attractive targets for further development of new antiviralcompounds.