Fusion of Pyruvate Decarboxylase and Alcohol Dehydrogenase Increases Ethanol Production in Escherichia coli
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- 作者：Aleksandra J. Lewicka ; Jan J. Lyczakowski ; Gavin Blackhurst ; Christiana Pashkuleva ; Kyle Rothschild-Mancinelli ; Dainius Tautvai拧as ; Harry Thornton ; Hugo Villanueva ; Weike Xiao ; Justinas Slikas ; Louise Horsfall ; Alistair Elfick ; Christopher French
- 刊名：ACS Synthetic Biology
- 出版年：2014
- 出版时间：December 19, 2014
- 年：2014
- 卷：3
- 期：12
- 页码：976-978
- 全文大小：184K
- ISSN：2161-5063
文摘
Ethanol is an important biofuel. Heterologous expression of Zymomonas mobilis pyruvate decarboxylase (Pdc) and alcohol dehydrogenase (AdhB) increases ethanol production in Escherichia coli. A fusion of PDC and ADH was generated and expressed in E. coli. The fusion enzyme was demonstrated to possess both activities. AdhB activity was significantly lower when fused to PDC than when the two enzymes were expressed separately. However, cells expressing the fusion protein generated ethanol more rapidly and to higher levels than cells coexpressing Pdc and AdhB, suggesting a specific rate enhancement due to the fusion of the two enzymes.