Screening of Carotenoids in Tomato Fruits by Using Liquid Chromatography with Diode Array鈥揕inear Ion Trap Mass Spectrometry Detection

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• 作者：Alessandra Gentili ; Fulvia Caretti ; Salvatore Ventura ; Virginia P茅rez-Fern谩ndez ; Alessandro Venditti ; Roberta Curini
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2015
• 出版时间：August 26, 2015
• 年：2015
• 卷：63
• 期：33
• 页码：7428-7439
• 全文大小：488K
• ISSN：1520-5118

文摘

This paper presents an analytical strategy for a large-scale screening of carotenoids in tomato fruits by exploiting the potentialities of the triple quadrupole鈥搇inear ion trap hybrid mass spectrometer (QqQ_LIT). The method involves separation on C₃₀ reversed-phase column and identification by means of diode array detection (DAD) and atmospheric pressure chemical ionization鈥搈ass spectrometry (APCI-MS). The authentic standards of six model compounds were used to optimize the separative conditions and to predict the chromatographic behavior of untargeted carotenoids. An information dependent acquisition (IDA) was performed with (i) enhanced-mass scan (EMS) as the survey scan, (ii) enhanced-resolution (ER) scan to obtain the exact mass of the precursor ions (16鈥?5 ppm), and (iii) enhanced product ion (EPI) scan as dependent scan to obtain structural information. LC-DAD-multiple reaction monitoring (MRM) chromatograms were also acquired for the identification of targeted carotenoids occurring at low concentrations; for the first time, the relative abundance between the MRM transitions (ion ratio) was used as an extra tool for the MS distinction of structural isomers and the related families of geometrical isomers. The whole analytical strategy was high-throughput, because a great number of experimental data could be acquired with few analytical steps, and cost-effective, because only few standards were used; when applied to characterize some tomato varieties (鈥楾angerine鈥? 鈥楶achino鈥? 鈥楧atterino鈥? and 鈥楥amone鈥? and passata of 鈥楽an Marzano鈥?tomatoes, our method succeeded in identifying up to 44 carotenoids in the 鈥楾angerine鈥欌€?variety.