Anti-伪-glucosidase and Anti-dipeptidyl Peptidase-IV Activities of Extracts and Purified Compounds from Vitis thunbergii var. taiwaniana

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• 作者：Yin-Shiou Lin ; Chiy-Rong Chen ; Wei-Hau Wu ; Chi-Luan Wen ; Chi-I Chang ; Wen-Chi Hou
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2015
• 出版时间：July 22, 2015
• 年：2015
• 卷：63
• 期：28
• 页码：6393-6401
• 全文大小：517K
• ISSN：1520-5118

文摘

Ethanol extracts (Et) from the stem (S) and leaf (L) of Vitis thunbergii var. taiwaniana (VTT) were used to investigate yeast 伪-glucosidase and porcine kidney dipeptidyl peptidase-IV (DPP-IV) inhibitory activities. Both VTT-Et showed complete 伪-glucosidase inhibition at 0.1 mg/mL; VTT-S-Et and VTT-L-Et showed 26 and 11% DPP-IV inhibition, respectively, at 0.5 mg/mL. The VTT-Et interventions (20 and 50 mg/kg) resulted in improvements in impaired glucose tolerance of diet-induced obese rats. (+)-Hopeaphenol, (+)-vitisin A, and (鈭?-vitisin B were isolated from the ethyl acetate fractions of S-Et and showed yeast 伪-glucosidase inhibition (IC<sub>50sub> = 18.30, 1.22, and 1.02 渭M) and porcine kidney DPP-IV inhibition (IC<sub>50sub> = 401, 90.75, and 15.3 渭M) compared to acarbose (6.39 mM) and sitagliptin (47.35 nM), respectively. Both (+)-vitisin A and (鈭?-vitisin B showed mixed noncompetitive inhibition against yeast 伪-glucosidase and porcine kidney DPP-IV, respectively. These results proposed that VTT extracts might through inhibitions against 伪-glucosidase and DPP-IV improve the impaired glucose tolerance in diet-induced obese rats.