Optimization of Extraction of Lipase from Wheat Seeds (Triticum aestivum) by Response Surface Methodology
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- 作者：Morgana K. Pierozan ; Roger J. da Costa ; Octvio A. C. Antunes ; Enrique G. Oestreicher ; J. Vladimir Oliveira ; Rogrio L. Cansian ; Helen Treichel ; Dbora de Oliveira
- 刊名：Journal of Agricultural and Food Chemistry
- 出版年：2009
- 出版时间：October 28, 2009
- 年：2009
- 卷：57
- 期：20
- 页码：9716-9721
- 全文大小：811K
- 年卷期：v.57,no.20(October 28, 2009)
- ISSN：1520-5118
文摘
This work aimed to evaluate the effects of particle size, solid/solvent ratio (w/v), and reaction time on hydrolytic and esterification activities of a lipase extract from wheat seeds. The higher hydrolytic activity was 5.9 U/g with a particle size of 425 μm, solid/solvent ratio of 30:60 (w/v), and reaction time of 15 h, with maximum hydrolytic activity of 14.47 U/g after 24 h of precipitation. For esterification activity the best result was 57.88 U/g with a particle size of 425 μm and solid/solvent ratio of 30:120 (w/v) for 5 h, with a maximum value after 10 h of precipitation reaching 208.20 U/g. The partial characterization showed that the optimal pH and temperature were found to be 5.5 and 32−37 °C, respectively. The extract stability at low temperatures was kept after 48 h of storage in terms of esterification activity. The hydrolytic activity was kept constant at −10 °C during 72 h and diminished considerably after 24 h at 4 °C.