Poly(N-isopropylacrylamide)-Stabilized Gold Nanoparticles in Combination with Tricationic Branched Phenylene-Ethynylene Fluorophore for Protein Identification

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• 作者：Keerati Kusolkamabot ; Pornpen Sae-ung ; Nakorn Niamnont ; Kanet Wongravee ; Mongkol Sukwattanasinitt ; Voravee P. Hoven
• 刊名：Langmuir
• 出版年：2013
• 出版时间：October 1, 2013
• 年：2013
• 卷：29
• 期：39
• 页码：12317-12327
• 全文大小：563K
• 年卷期：v.29,no.39(October 1, 2013)
• ISSN：1520-5827

文摘

Gold nanoparticles stabilized by thermoresponsive polymer, poly(N-isopropylacrylamide) (PNIPAM-AuNPs) were prepared by surface grafting of thiol-terminated PNIPAM onto citrate-stabilized AuNPs. The color change of the PNIPAM-AuNPs solution from red to blue-purple without precipitation when the solution was heated to 40 掳C, above the lower critical solution temperature (LCST) of PNIPAM, indicated the thermoresponsive property of the synthesized AuNPs. PNIPAM-AuNPs were used to detect proteins by chemical nose approach based on fluorescence quenching of fluorophore by AuNPs. An array-based sensing platform for detection of six proteins, namely bovine serum albumin, lysozyme, fibrinogen, concanavalin A, hemoglobin, holo-transferrin human can be successfully developed from the PNIPAM-AuNPs having different molecular weights (4 and 8 kDa) and conformation (varied heat treatment from 25 to 40 掳C) in combination with a tricationic branched phenylene-ethynylene fluorophore. From principal component analysis (PCA) followed by linear discriminant analysis (LDA), 100% accuracy of protein classification using a leave-one-out (LOO) approach can be achieved by using only two types of PNIPAM-AuNPs.