Antiviral Phagocytosis Is Regulated by a Novel Rab-Dependent Complex in Shrimp Penaeus japonicus

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• 作者：Wenlin Wu ; Rongrong Zong ; Jianyang Xu ; Xiaobo Zhang
• 刊名：Journal of Proteome Research
• 出版年：2008
• 出版时间：January 2008
• 年：2008
• 卷：7
• 期：1
• 页码：424 - 431
• 全文大小：1083K
• 年卷期：v.7,no.1(January 2008)
• ISSN：1535-3907

文摘

Rab GTPases are involved in phagosome formation and maturation. However, the role of Rab GTPases in phagocytosis against virus infection remains unknown. In this study, it was found that a Rab gene (PjRab) from marine shrimp was upregulated in virus-resistant shrimp, suggesting that Rab GTPase was involved in the innate response to virus. The RNAi and mRNA assays revealed that the PjRab protein could regulate shrimp hemocytic phagocytosis through a protein complex consisting of the PjRab, β-actin, tropomyosin, and envelope protein VP466 of shrimp white spot syndrome virus (WSSV). It was further demonstrated that the PjRab gene silencing by RNAi caused the increase in the number of WSSV copies, indicating that the PjRab might be an intracellular virus recognition protein employed by a host to increase the phagocytic activity. Therefore, our study presents a novel Rab-dependent signaling complex, in which the Rab GTPase might detect virus infection as an intracellular virus recognition protein and trigger downstream phagocytic defense against virus in crustacean for the first time. This discovery would improve our understanding of the still poorly understood molecular events involved in innate immune response against virus infection of invertebrates.