Evolution of Culture Broth Rheological Properties during Propagation of the Entomopathogenic Nematode Steinernema carpocapsae, in Submerged Monoxenic Culture
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- 作者：Norberto Chavarrí ; a-Herná ; ndez ; Adriana Iné ; s Rodrí ; guez-Herná ; ndez ; Fermí ; n Pé ; rez-Guevara ; and Mayra de la Torre
- 刊名：Biotechnology Progress
- 出版年：2003
- 出版时间：April 2003
- 年：2003
- 卷：19
- 期：2
- 页码：405 - 409
- 全文大小：183K
- 年卷期：v.19,no.2(April 2003)
- ISSN：1520-6033
文摘
This article presents the evolution of culture broth rheological properties duringmonoxenic cultures of Steinernema carpocapsae in cylindrical bottles agitated orbitally.Rheological properties were evaluated in simple-shear flow conditions and were well-modeled by the Ostwald-de Waele model. Rheological properties varied from slightlydilatant, n = 1.2 (-), to moderately pseudoplastic flow behavior, n = 0.6 (-). Nematodeconcentrations increased from 750 ± 190 to 130 900 ± 6900 nematodes/mL, and theapparent viscosity (
a) evolved from 4.5 ± 0.7 to 46.6 ± 3.2 mPa·s during thefermentations. Rheological behavior did not appear to be strongly influenced bynematode number and/or its stage of development; however, the release of substancesfrom the decomposition of nematode cadavers appeared to be of great importance.Among the different developmental stages of the nematodes, only juveniles of the firststage (J1) were highly susceptible to the shearing conditions tested (shear stress, 
r
,from 0.9 to 3.5 Pa during periods of 80-100 min), resulting in the viability loss of85% of J1 nematodes.
a) evolved from 4.5 ± 0.7 to 46.6 ± 3.2 mPa·s during thefermentations. Rheological behavior did not appear to be strongly influenced bynematode number and/or its stage of development; however, the release of substancesfrom the decomposition of nematode cadavers appeared to be of great importance.Among the different developmental stages of the nematodes, only juveniles of the firststage (J1) were highly susceptible to the shearing conditions tested (shear stress, r,from 0.9 to 3.5 Pa during periods of 80-100 min), resulting in the viability loss of85% of J1 nematodes.