文摘
Fluorescence detection and imaging are vital technologies in the life sciences and clinicaldiagnostics. The key to obtaining high-resolution images and sensitive detection is to use fluorescentmolecules or particles that absorb and emit visible light with high efficiency. We have synthesizedsupramolecular complexes consisting of a branched DNA template and fluorogenic intercalating dyes.Because dyes can intercalate up to every other base pair, high densities of fluorophores are assembledyet the DNA template keeps them far enough away from each other to prevent self-quenching. The efficiencywith which these noncovalent assemblies absorb light is more than 10-fold greater than that of the individualdye molecules. Förster resonance energy transfer from the intercalated dyes to covalently attached acceptordyes is very efficient, allowing for wavelength shifting of the emission spectrum. Simple biotinylation of theDNA template allows for labeling of streptavidin-coated synthetic microspheres and mouse T-cells.