Stearylated Arginine-Rich Peptides: A New Class of Transfection Systems

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• 作者：Shiroh Futaki ; Wakana Ohashi ; Tomoki Suzuki ; Miki Niwa ; Seigo Tanaka ; Kunihiro Ueda ; Hideyoshi Harashima ; and Yukio Sugiura
• 刊名：Bioconjugate Chemistry
• 出版年：2001
• 出版时间：November 2001
• 年：2001
• 卷：12
• 期：6
• 页码：1005 - 1011
• 全文大小：101K
• 年卷期：v.12,no.6(November 2001)
• ISSN：1520-4812

文摘

Membrane-permeable arginine-rich peptides, such as HIV-1 Tat-(48-60), HIV-1 Rev-(34-50), andflock house virus (FHV) coat-(35-49), have been shown to possess the ability to transfect COS-7 cellswith luciferase-coding plasmid as efficiently as polyarginine (MW 5000-15 000) and polylysine (MW9800). Not only these virus-derived cationic peptides but also oligoarginines of 4-16 residues werefound to be able to transfect cells. In the case of the Tat, FHV, and octaarginine peptides, N-terminalstearylation of the peptides increases the transfection efficiency by approximately 100 times to reachthe same order of magnitude as that of LipofectAMINE, one of the most efficient commercially availabletransfection agents. Also, a certain correlation was observed between the transfection efficiency ofstearyl-(Arg)_n peptides (stearyl-R_n: n = 4, 8, 12, 16) and the membrane permeability of thecorresponding (Arg)_n peptides (R_n).