Imaging Multiple Conductance States in an Alamethicin Pore

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• 作者：Lydia M. Harriss ; Br铆d Cronin ; James R. Thompson ; Mark I. Wallace
• 刊名：Journal of the American Chemical Society
• 出版年：2011
• 出版时间：September 21, 2011
• 年：2011
• 卷：133
• 期：37
• 页码：14507-14509
• 全文大小：728K
• 年卷期：v.133,no.37(September 21, 2011)
• ISSN：1520-5126

文摘

Alamethicin is the archetypal antimicrobial pore-forming peptide. Although the peptide has long been known to form pores of characteristic conductances in lipid membranes, the precise nature of these pores is not known. Simultaneous calcium-flux imaging and single-channel recording in a droplet interface bilayer allowed us to directly attribute multiple conductance states to a single point diffusing in the bilayer.