Multifunctional Dendrimer-Modified Multiwalled Carbon Nanotubes: Synthesis, Characterization, and In Vitro Cancer Cell Targeting and Imaging

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• 作者：Xiangyang Shi ; Su He Wang ; Mingwu Shen ; Mary E. Antwerp ; Xisui Chen ; Chang Li ; Elijah J. Petersen ; Qingguo Huang ; Walter J. Weber ; Jr. ; James R. Baker ; Jr.
• 刊名：Biomacromolecules
• 出版年：2009
• 出版时间：July 13, 2009
• 年：2009
• 卷：10
• 期：7
• 页码：1744-1750
• 全文大小：318K
• 年卷期：v.10,no.7(July 13, 2009)
• ISSN：1526-4602

文摘

Carbon nanotubes hold great promise for their use as a platform in nanomedicine, especially in drug delivery, medical imaging, and cancer targeting and therapeutics. Herein, we present a facile approach to modifying carbon nanotubes with multifunctional poly(amidoamine) (PAMAM) dendrimers for cancer cell targeting and imaging. In this approach, fluorescein isothiocyanate (FI)- and folic acid (FA)-modified amine-terminated generation 5 (G5) PAMAM dendrimers (G5·NH₂-FI-FA) were covalently linked to acid-treated multiwalled carbon nanotubes (MWCNTs), followed by acetylation of the remaining primary amine groups of the dendrimers. The resulting MWCNT/G5.NHAc-FI-FA composites are water-dispersible, stable, and biocompatible. In vitro flow cytometry and confocal microscopy data show that the formed MWCNT/G5·NHAc-FI-FA composites can specifically target to cancer cells overexpressing high-affinity folic acid receptors. The results of this study suggest that, through modification with multifunctional dendrimers, complex carbon nanotube-based materials can be fabricated, thereby providing many possibilities for various applications in biomedical sensing, diagnosis, and therapeutics.