Dendrimeric Bowties Featuring Hemispheric-Selective Decoration of Ligands for microRNA-Based Therapy

详细信息    查看全文

• 作者：Warren D. Gray ; Ruo Jia Wu ; Xiang Yin ; Jihan Zhou ; Michael E. Davis ; Ying Luo
• 刊名：Biomacromolecules
• 出版年：2013
• 出版时间：January 14, 2013
• 年：2013
• 卷：14
• 期：1
• 页码：101-109
• 全文大小：444K
• 年卷期：v.14,no.1(January 14, 2013)
• ISSN：1526-4602

文摘

Dendrimers feature a defined number of terminal groups that may bind RNA or be functionalized with bioactive molecules. These competing uses of terminal groups may create an impasse if the requisite density of ligands depletes the number of terminal groups for binding sufficient RNA, or vice versa. A novel dendrimeric platform is needed that maintains high ligand density while retaining sufficient microRNA-binding terminal groups. Here we present a dendrimeric 鈥渂owtie鈥?consisting of one-half devoted to microRNA binding and the other half to ligand presentation. We demonstrate its suitability as a transfection agent by delivering miR-126 to human vascular endothial cells (HUVECs) via polyarginine- and RGD-modified bowties and evaluating the downstream effects on proliferation and tube formation. Our findings indicate that the bowtie elicits desired responses and may possess superior delivery properties compared to nondecorated dendrimeric materials. The bowtie system thereby provides a new design model for developing dendrimeric delivery vehicles for RNAi therapeutics.