Human Mesenchymal Stem Cells Tissue Development in 3D PET Matrices
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- 作者：Warren L. Grayson ; Teng Ma ; and Bruce Bunnell
- 刊名：Biotechnology Progress
- 出版年：2004
- 出版时间：June 2004
- 年：2004
- 卷：20
- 期：3
- 页码：905 - 912
- 全文大小：745K
- 年卷期：v.20,no.3(June 2004)
- ISSN：1520-6033
文摘
Human mesenchymal stem cells (hMSCs) are attractive cell sources for engineeredtissue constructs with broad therapeutic potential. Three-dimensional (3D) hMSCtissue development in nonwoven poly(ethylene terephthalate) (PET) fibrous matriceswas investigated. HMSCs were seeded onto 3D PET scaffolds and were cultured forover 1 month. Their proliferation rates were affected by seeding density but remainedmuch lower than those of 2D controls. Compared to 2D surfaces, hMSCs grown in 3Dscaffolds secreted and embedded themselves in an extensive ECM network composedof collagen I, collagen IV, fibronectin, and laminin. HMSCs were influenced by theorientation of adjacent PET fibers to organize the ECM proteins into highly alignedfibrils. We observed the increased expressions of 
2
ddle">1 integrin but a slight decreasein the expression of 5ddle">1 integrin in 3D compared to 2D culture and found that Vddle">3was expressed only in 2D. Paxillin expression was down-regulated in 3D culture witha concomitant change in its localization patterns. We demonstrated the multi-lineagepotentials of the 3D tissue constructs by differentiating the cells grown in the scaffoldsinto osteoblasts and adipocytes. Taken together, these results showed that hMSCsgrown in 3D scaffolds display tissue development patterns distinct from their 2Dcounterparts and provide important clues for designing 3D scaffolds for developingtissue engineered constructs.
2ddle">1 integrin but a slight decreasein the expression of 5ddle">1 integrin in 3D compared to 2D culture and found that Vddle">3was expressed only in 2D. Paxillin expression was down-regulated in 3D culture witha concomitant change in its localization patterns. We demonstrated the multi-lineagepotentials of the 3D tissue constructs by differentiating the cells grown in the scaffoldsinto osteoblasts and adipocytes. Taken together, these results showed that hMSCsgrown in 3D scaffolds display tissue development patterns distinct from their 2Dcounterparts and provide important clues for designing 3D scaffolds for developingtissue engineered constructs.