Role of the HoxZ Subunit in the Electron Transfer Pathway of the Membrane-Bound [NiFe]-Hydrogenase from Ralstonia eutropha Immobilized on Electrodes

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• 作者：Murat Sezer ; Stefan Frielingsdorf ; Diego Millo ; Nina Heidary ; Tillman Utesch ; Maria-Andrea Mroginski ; Ba虉rbel Friedrich ; Peter Hildebrandt ; Ingo Zebger ; Inez M. Weidinger
• 刊名：Journal of Physical Chemistry B
• 出版年：2011
• 出版时间：September 1, 2011
• 年：2011
• 卷：115
• 期：34
• 页码：10368-10374
• 全文大小：847K
• 年卷期：v.115,no.34(September 1, 2011)
• ISSN：1520-5207

文摘

The role of the diheme cytochrome b (HoxZ) subunit in the electron transfer pathway of the membrane-bound [NiFe]-hydrogenase (MBH) heterotrimer from Ralstonia eutropha H16 has been investigated. The MBH in its native heterotrimeric state was immobilized on electrodes and subjected to spectroscopic and electrochemical analysis. Surface enhanced resonance Raman spectroscopy was used to monitor the redox and coordination state of the HoxZ heme cofactors while concomitant protein film voltammetric measurements gave insights into the catalytic response of the enzyme on the electrode. The entire MBH heterotrimer as well as its isolated HoxZ subunit were immobilized on silver electrodes coated with self-assembled monolayers of 蠅-functionalized alkylthiols, displaying the preservation of the native heme pocket structure and an electrical communication between HoxZ and the electrode. For the immobilized MBH heterotrimer, catalytic reduction of the HoxZ heme cofactors was observed upon H₂ addition. The catalytic currents of MBH with and without the HoxZ subunit were measured and compared with the heterogeneous electron transfer rates of the isolated HoxZ. On the basis of the spectroscopic and electrochemical results, we conclude that the HoxZ subunit under these artificial conditions is not primarily involved in the electron transfer to the electrode but plays a crucial role in stabilizing the enzyme on the electrode.