Reaction-Based “Off–On‿Fluorescent Probe Enabling Detection of Endogenous Labile Fe2+ and Imaging of Zn2+-induced Fe2+ Flux in Living Cells and Elevated Fe2+ in Ischemic Stroke

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• 作者：Weimin Xuan ; Rong Pan ; Yongyi Wei ; Yanting Cao ; Huanqiu Li ; Fu-Sen Liang ; Ke-Jian Liu ; Wei Wang
• 刊名：Bioconjugate Chemistry
• 出版年：2016
• 出版时间：February 17, 2016
• 年：2016
• 卷：27
• 期：2
• 页码：302-308
• 全文大小：437K
• ISSN：1520-4812

文摘

Fluorogenic sensors capable of spatiotemporally detecting Fe²⁺ in biological systems are highly valuable in the study of iron biology. Toward this end, a new “off–on” Fe²⁺-selective fluorescent probe has been developed by incorporating an Fe²⁺-induced N–O cleavage of acylated hydroxylamine moiety into the naphthalimide fluorophore. The probe displays facile response (within 15 min) and good selectivity toward Fe²⁺ with >27-fold enhancement of fluorescence intensity and high sensitivity of as low as 0.5 μM with a noticeable 3-fold fluorescence enhancement. These features of the probe have been transformed into in the convenient detection of endogenous, basal level of labile Fe²⁺ pools in living cells. Furthermore, we have demonstrated the capacity of the probe for the studies of important Fe²⁺ related biological functions. It can respond to the Zn²⁺-induced Fe²⁺ flux, an important event observed in stroke, and facilely detect the elevated level of Fe²⁺ in the brain tissue of a rat undergoing ischemic stroke at the ischemic site.