Detection of Tumor Invasive Biomarker using a Peptamer of Signal Conversion and Signal Amplification

详细信息    查看全文

• 作者：Hao Li ; Weiwei Li ; Fengzhen Liu ; Zhaoxia Wang ; Genxi Li ; Yannis Karamanos
• 刊名：Analytical Chemistry
• 出版年：2016
• 出版时间：April 5, 2016
• 年：2016
• 卷：88
• 期：7
• 页码：3662-3668
• 全文大小：480K
• ISSN：1520-6882

文摘

Inspired by the structural and functional features of proteins in cell signaling, a switchable peptide is designed in this work. This switchable peptide is named a “peptamer,” and it can react to ligand binding with conformational change and activation/deactivation of catalytic ability. The peptamer is constructed by elaborately integrating several different peptide motifs with targeting and catalytic abilities. Thus, targeted binding of the peptamer to an integrin can be regulated by a synthetic ligand. Moreover, the conformational rearrangement of the peptamer induced by both integrin and the synthetic ligand can resolve in altered affinity of the peptamer for a catalytic cofactor, cupric ion. This leads to greatly contrasted efficiency of catalysis in the presence/absence of integrin. This distinct switching on/off of catalytic activity also enables a bioassay of tissue integrin expression in clinical samples of thyroid carcinoma. Experimental results reveal that the detected integrin level parallels the state of lymph node metastasis. Therefore, this simple peptide model may help to understand the structural reconfiguration of proteins involved in cellular signal transduction, as well as to provide a new means to assess protein activity under pathological conditions such as cancer.