Specific Gene Repression by CRISPRi System Transferred through Bacterial Conjugation
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- 作者：Weiyue Ji ; Derrick Lee ; Eric Wong ; Priyanka Dadlani ; David Dinh ; Verna Huang ; Kendall Kearns ; Sherry Teng ; Susan Chen ; John Haliburton ; Graham Heimberg ; Benjamin Heineike ; Anusuya Ramasubramanian ; Thomas Stevens ; Kara J. Helmke ; Veronica Zepeda ; Lei S. Qi ; Wendell A. Lim
- 刊名：ACS Synthetic Biology
- 出版年：2014
- 出版时间：December 19, 2014
- 年：2014
- 卷：3
- 期：12
- 页码：929-931
- 全文大小：226K
- ISSN：2161-5063
文摘
In microbial communities, bacterial populations are commonly controlled using indiscriminate, broad range antibiotics. There are few ways to target specific strains effectively without disrupting the entire microbiome and local environment. Here, we use conjugation, a natural DNA horizontal transfer process among bacterial species, to deliver an engineered CRISPR interference (CRISPRi) system for targeting specific genes in recipient Escherichia coli cells. We show that delivery of the CRISPRi system is successful and can specifically repress a reporter gene in recipient cells, thereby establishing a new tool for gene regulation across bacterial cells and potentially for bacterial population control.