Synthesis and Application of a Water-Soluble Near-Infrared Dye for Cancer Detection Using Optical Imaging

详细信息    查看全文

• 作者：Wellington Pham ; Zdravka Medarova ; and Anna Moore
• 刊名：Bioconjugate Chemistry
• 出版年：2005
• 出版时间：May 2005
• 年：2005
• 卷：16
• 期：3
• 页码：735 - 740
• 全文大小：270K
• 年卷期：v.16,no.3(May 2005)
• ISSN：1520-4812

文摘

A novel water-soluble 2-[2-(2-chloro-3-{2-[3,3-dimethyl-5-sulfo-1-(4-sulfo-butyl)-3H-indol-2-yl]-vinyl}-cyclohex-2-enylidene)-ethylidene]-3,3-dimethyl-1-(4-sulfo-butyl)-2,3-dihydro-1H-indole-5-carboxylic acid(dye 2) was developed via an asymmetric approach. With an additional sulfonate group, the near-infrared feature of this dye exhibited a 2-fold increase in quantum yield compared to the previousgeneration. The current synthetic strategy provided a single carboxylic group as a handle forconjugation, thus allowing selectivity for bioconjugation. The stability of this dye was demonstratedby labeling peptides via solid-phase peptide chemistry. The in vivo optical imaging showed potentialand broad applications of this dye in developing molecular-based beacons for cancer detection.