Selective, Highly Sensitive Fluorescent Probe for the Detection of Sulfur Dioxide Derivatives in Aqueous and Biological Environments

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• 作者：Wenqiang Chen ; Qian Fang ; Dalei Yang ; Hongyan Zhang ; Xiangzhi Song ; James Foley
• 刊名：Analytical Chemistry
• 出版年：2015
• 出版时间：January 6, 2015
• 年：2015
• 卷：87
• 期：1
• 页码：609-616
• 全文大小：460K
• ISSN：1520-6882

文摘

On the basis of a unique nucleophilic addition reaction, a novel water-soluble broadly emitting (500鈥?00 nm) fluorescent Probe 1 was developed for the rapid detection of SO₂ derivatives in aqueous media. The positively-charged benzopyrylium moiety in Probe 1 provides both excellent water solubility, making this probe applicable in 100% aqueous environments, and the ability to function as a fluorescence quencher of the coumarin moiety. Probe 1 generates a nearly instantaneous strong fluorescence signal in response to SO₂ derivatives having an 8.3 nM detection limit for bisufite. The resulting Probe 1鈥搒ulfite adduct emits in the green/red spectral region (位_max = 585 nm) with a large Stokes shift (139 nm). The probe exhibits excellent selectivity toward SO₂ derivatives over other potential interfering agents including reactive sulfur-containing species. Importantly, we demonstrate that Probe 1 can be used for the real-time sensing and bioimaging of SO₂ derivatives in living cells.