Quantification of Proteins by Functionalized Gold Nanoparticles Using Click Chemistry
详细信息 查看全文
- 作者：Kui Zhu ; Yi Zhang ; Sha He ; Wenwen Chen ; Jianzhong Shen ; Zhuo Wang ; Xingyu Jiang
- 刊名：Analytical Chemistry
- 出版年：2012
- 出版时间：May 15, 2012
- 年：2012
- 卷：84
- 期：10
- 页码：4267-4270
- 全文大小：218K
- 年卷期：v.84,no.10(May 15, 2012)
- ISSN：1520-6882
文摘
This letter presents a click-chemistry-based assay for proteins (CAP) that allows quantitative determination of the concentration of proteins, using azide- and alkyne-functionalized gold nanoparticles (AuNPs). Compared with conventional methods, CAP has a broader linear range for detection of proteins with good selectivity. CAP enables the analysis of total proteins in various sera and milk samples.