Copolythiophene-Derived Colorimetric and Fluorometric Sensor for Visually Supersensitive Determination of Lipopolysaccharide

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• 作者：Minhuan Lan ; Jiasheng Wu ; Weimin Liu ; Wenjun Zhang ; Jiechao Ge ; Hongyan Zhang ; Jiayu Sun ; Wenwen Zhao ; Pengfei Wang
• 刊名：The Journal of the American Chemical Society
• 出版年：2012
• 出版时间：April 18, 2012
• 年：2012
• 卷：134
• 期：15
• 页码：6685-6694
• 全文大小：591K
• 年卷期：v.134,no.15(April 18, 2012)
• ISSN：1520-5126

文摘

3-Phenylthiophene-based water-soluble copolythiophenes (CPT1) were designed for colorimetric and fluorometric detection of lipopolysaccharide (LPS). The sensor (CPT1-C) shows a high selectivity to LPS in the presence of other negatively charged bioanalytes as well an extreme sensitivity with the detection limit at picomolar level, which is the lowest ever achieved among all synthetic LPS sensors available thus far. Significantly, the sensing interaction can be apparently observed by the naked eyes, which presents a great advantage for its practical applications. The appealing performance of sensor was demonstrated to originate from the multiple electrostatic and hydrophobic cooperative interactions, synergetic with signal amplification via the conformational change of the 3-phenylthiophene-based copolymer main chain. As a straightforward application, CPT1-C is capable of rapidly discriminating the Gram-negative bacteria (with LPS in the membrane) from Gram-positive bacteria (without LPS).