Fine Particulate Matter Constituents, Nitric Oxide Synthase DNA Methylation and Exhaled Nitric Oxide

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• 作者：Renjie Chen ; Liping Qiao ; Huichu Li ; Yan Zhao ; Yunhui Zhang ; Wenxi Xu ; Cuicui Wang ; Hongli Wang ; Zhuohui Zhao ; Xiaohui Xu ; Hui Hu ; Haidong Kan
• 刊名：Environmental Science & Technology
• 出版年：2015
• 出版时间：October 6, 2015
• 年：2015
• 卷：49
• 期：19
• 页码：11859-11865
• 全文大小：308K
• ISSN：1520-5851

文摘

It remains unknown how fine particulate matter (PM_2.5) constituents affect differently the fractional concentration of exhaled nitric oxide (FeNO, a biomarker of airway inflammation) and the DNA methylation of its encoding gene (NOS2A). We aimed to investigate the short-term effects of PM_2.5 constituents on NOS2A methylation and FeNO. We designed a longitudinal study among chronic obstructive pulmonary disease (COPD) patients with six repeated health measurements in Shanghai, China. We applied linear mixed-effect models to evaluate the associations. We observed that the inverse association between PM_2.5 and methylation at position 1 was limited within 24 h, and the positive association between PM_2.5 and FeNO was the strongest at lag 1 day. Organic carbon, element carbon, NO₃^{鈥?/sup> and NH₄+ were robustly and significantly associated with decreased methylation and elevated FeNO. An interquartile range increase in total PM_2.5 and the four constituents was associated with decreases of 1.19, 1.63, 1.62, 1.17, and 1.14 in percent methylation of NOS2A, respectively, and increases of 13.30%,16.93%, 8.97%, 18.26%, and 11.42% in FeNO, respectively. Our results indicated that organic carbon, element carbon, NO₃鈥?/sup> and NH₄+ might be mainly responsible for the effects of PM_2.5 on the decreased NOS2A DNA methylation and elevated FeNO in COPD patients.}