Chemical Characterization of the Smallest S-Nitrosothiol, HSNO; Cellular Cross-talk of H2S and S-Nitrosothiols

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• 作者：Milos R. Filipovic ; Jan Lj. Miljkovic ; Thomas Nauser ; Maksim Royzen ; Katharina Klos ; Tatyana Shubina ; Willem H. Koppenol ; Stephen J. Lippard ; Ivana Ivanovi膰-Burmazovi膰
• 刊名：The Journal of the American Chemical Society
• 出版年：2012
• 出版时间：July 25, 2012
• 年：2012
• 卷：134
• 期：29
• 页码：12016-12027
• 全文大小：559K
• 年卷期：v.134,no.29(July 25, 2012)
• ISSN：1520-5126

文摘

Dihydrogen sulfide recently emerged as a biological signaling molecule with important physiological roles and significant pharmacological potential. Chemically plausible explanations for its mechanisms of action have remained elusive, however. Here, we report that H₂S reacts with S-nitrosothiols to form thionitrous acid (HSNO), the smallest S-nitrosothiol. These results demonstrate that, at the cellular level, HSNO can be metabolized to afford NO⁺, NO, and NO^{鈥?/sup> species, all of which have distinct physiological consequences of their own. We further show that HSNO can freely diffuse through membranes, facilitating transnitrosation of proteins such as hemoglobin. The data presented in this study explain some of the physiological effects ascribed to H₂S, but, more broadly, introduce a new signaling molecule, HSNO, and suggest that it may play a key role in cellular redox regulation.}