Stability of (Bio)Functionalized Porous Aluminum Oxide

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• 作者：Aline Debrassi ; Angela Ribbera ; Willem M. de Vos ; Tom Wennekes ; Han Zuilhof
• 刊名：Langmuir
• 出版年：2014
• 出版时间：February 11, 2014
• 年：2014
• 卷：30
• 期：5
• 页码：1311-1320
• 全文大小：500K
• ISSN：1520-5827

文摘

Porous aluminum oxide (PAO), a nanostructured support for, among others, culturing microorganisms, was chemically modified in order to attach biomolecules that can selectively interact with target bacteria. We present the first comprehensive study of monolayer-modified PAO using conditions that are relevant to microbial growth with a range of functional groups (carboxylic acid, 伪-hydroxycarboxylic acid, alkyne, alkene, phosphonic acid, and silane). Their stability was initially assessed in phosphate-buffered saline (pH 7.0) at room temperature. The most stable combination (PAO with phosphonic acids) was further studied over a range of physiological pHs (4鈥?) and temperatures (up to 80 掳C). Varying the pH had no significant effect on the stability, but it gradually decreased with increasing temperature. The stability of phosphonic acid-modified PAO surfaces was shown to depend strongly on the other terminal group of the monolayer structure: in general, hydrophilic monolayers were less stable than hydrophobic monolayers. Finally, an alkyne-terminated PAO surface was reacted with an azide-linked mannose derivative. The resulting mannose-presenting PAO surface showed the clearly increased adherence of a mannose-binding bacterium, Lactobacillus plantarum, and also allowed for bacterial outgrowth.