Monitoring Poly(3-hydroxybutyrate) Production in Cupriavidus necator DSM 428 (H16) with Raman Spectroscopy

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• 作者：Joke De Gelder ; Diana Willemse-Erix ; Maarten J. Scholtes ; Jorge I. Sanchez ; Kees Maquelin ; Peter Vandenabeele ; Patrick De Boever ; Gerwin J. Puppels ; Luc Moens ; Paul De Vos
• 刊名：Analytical Chemistry
• 出版年：2008
• 出版时间：March 15, 2008
• 年：2008
• 卷：80
• 期：6
• 页码：2155 - 2160
• 全文大小：170K
• 年卷期：v.80,no.6(March 15, 2008)
• ISSN：1520-6882

文摘

This study explored the potential of Raman spectroscopyfor the analysis of poly(3-hydroxybutyrate) (PHB) inbacteria. PHB can be formed in large amounts by certainbacteria as a storage material and is of high importancefor industrial biodegradable plastic production. Ramanspectra were collected from Cupriavidus necator DSM428 (H16), from its non-PHB-producing mutant strainC. necator DSM 541, and from pure PHB, in order todetermine at which Raman shifts a contribution of PHBin bacterial spectra can be expected. The Raman bandintensity at ca. 1734 cm^-1 appeared to be suitable for themonitoring of PHB production and consumption. Theseintensities were linearly related to the PHB concentration(mg L^-1 culture) determined by parallel HPLC analysis.Therefore, Raman spectroscopy is considered as a fastand noninvasive technique for the determination andmonitoring of the PHB content in bacteria.