文摘
This study explored the potential of Raman spectroscopyfor the analysis of poly(3-hydroxybutyrate) (PHB) inbacteria. PHB can be formed in large amounts by certainbacteria as a storage material and is of high importancefor industrial biodegradable plastic production. Ramanspectra were collected from Cupriavidus necator DSM428 (H16), from its non-PHB-producing mutant strainC. necator DSM 541, and from pure PHB, in order todetermine at which Raman shifts a contribution of PHBin bacterial spectra can be expected. The Raman bandintensity at ca. 1734 cm-1 appeared to be suitable for themonitoring of PHB production and consumption. Theseintensities were linearly related to the PHB concentration(mg L-1 culture) determined by parallel HPLC analysis.Therefore, Raman spectroscopy is considered as a fastand noninvasive technique for the determination andmonitoring of the PHB content in bacteria.