DNAzyme-Controlled Cleavage of Dimer and Trimer Origami Tiles

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• 作者：Na Wu ; Itamar Willner
• 刊名：Nano Letters
• 出版年：2016
• 出版时间：April 13, 2016
• 年：2016
• 卷：16
• 期：4
• 页码：2867-2872
• 全文大小：488K
• 年卷期：0
• ISSN：1530-6992

文摘

Dimers of origami tiles are bridged by the Pb²⁺-dependent DNAzyme sequence and its substrate or by the histidine-dependent DNAzyme sequence and its substrate to yield the dimers T₁–T₂ and T₃–T₄, respectively. The dimers are cleaved to monomer tiles in the presence of Pb²⁺-ions or histidine as triggers. Similarly, trimers of origami tiles are constructed by bridging the tiles with the Pb²⁺-ion-dependent DNAzyme sequence and the histidine-dependent DNAzyme sequence and their substrates yielding the trimer T₁–T₅–T₄. In the presence of Pb²⁺-ions and/or histidine as triggers, the programmed cleavage of trimer proceeds. Using Pb²⁺ or histidine as trigger cleaves the trimer to yield T₅–T₄ and T₁ or the dimer T₁–T₅ and T₄, respectively. In the presence of Pb²⁺-ions and histidine as triggers, the cleavage products are the monomer tiles T₁, T₅, and T₄. The different cleavage products are identified by labeling the tiles with 0, 1, or 2 streptavidin labels and AFM imaging.