Adding Gas Chromatography鈥揗ass Spectrometry Data to a Melting Point and Thin-Layer Chromatography Laboratory
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- 作者：Adam M. Azman ; Julie A. Barrett ; Megan Darragh ; John J. Esteb ; LuAnne M. McNulty ; Paul M. Morgan ; Stacy A. O鈥橰eilly ; Anne M. Wilson
- 刊名：Journal of Chemical Education
- 出版年：2013
- 出版时间：January 8, 2013
- 年：2013
- 卷：90
- 期：1
- 页码：140-141
- 全文大小：128K
- 年卷期：v.90,no.1(January 8, 2013)
- ISSN：1938-1328
文摘
The addition of gas chromatography鈥搈ass spectrometry (GC鈥揗S) data interpretation to a thin-layer chromatography (TLC) and melting point (mp) laboratory for an introductory organic course is described. Students are given a sample containing one unknown and a list of 24 unknown compounds, organized in groups by similar mp ranges. Students identify the unknown through mp and TLC. Students are then given GC鈥揗S data containing three unknowns and are asked to determine GC peak corresponding to their unknown using the MS spectra. Upon completion of the laboratory, students have gained an understanding of how many components are present given GC鈥揗S data and how to identify each component from the molecular ion. They also become familiar with the physical characteristics of melting point, retention factor, and retention time. These skills are utilized in other experiments throughout the academic year