Tracking Bismuth Antiulcer Drug Uptake in Single Helicobacter pylori Cells
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- 作者：Cheuk-Nam Tsang ; Koon-Sing Ho ; Hongzhe Sun ; Wing-Tat Chan
- 刊名：Journal of the American Chemical Society
- 出版年：2011
- 出版时间：May 18, 2011
- 年：2011
- 卷：133
- 期：19
- 页码：7355-7357
- 全文大小：732K
- 年卷期：v.133,no.19(May 18, 2011)
- ISSN：1520-5126
文摘
Bismuth-based drugs have long been used for the treatment of Helicobacter pylori infection. In this work, the metal content in H. pylori was monitored at the single-cell level by time-resolved inductively coupled plasma mass spectrometry, and 2.9 脳 107 Mg atoms/cell was determined for the wild-type. Bacteria treated with a Bi antiulcer drug deposited nearly 1.0 脳 106 Bi atoms/cell, whereas the uptake process took 3 h to reach the half-maximum. Interference of ferric ions on bismuth uptake was demonstrated, suggesting that the metallodrug can utilize certain iron-transport pathways in the pathogen. The approach provides a general strategy for monitoring metals in single cells, facilitating exploration of metal-relevant bioprocesses.