DNA Concentration Modulation on Supported Lipid Bilayers Switched by Surface Acoustic Waves

详细信息    查看全文

• 作者：Martin Hennig ; Manuel Wolff ; J眉rgen Neumann ; Achim Wixforth ; Matthias F. Schneider ; Joachim O. R盲dler
• 刊名：Langmuir
• 出版年：2011
• 出版时间：December 20, 2011
• 年：2011
• 卷：27
• 期：24
• 页码：14721-14725
• 全文大小：844K
• 年卷期：v.27,no.24(December 20, 2011)
• ISSN：1520-5827

文摘

Spatially addressable arrays of molecules embedded in or anchored to supported lipid bilayers are important for on-chip screening and binding assays; however, methods to sort or accumulate components in a fluid membrane on demand are still limited. Here we apply in-plane surface acoustic shear waves (SAWs) to laterally accumulate double-stranded DNA segments electrostatically bound to a cationic supported lipid bilayer. The fluorescently labeled DNA segments are found to segregate into stripe patterns with a spatial frequency corresponding to the periodicity of the standing SAW wave (10 渭m). The DNA molecules are accumulated 10-fold in the regions of SAW antinodes. The superposition of two orthogonal sets of SAW sources creates checkerboard like arrays of DNA demonstrating the potential to generate arrayed fields dynamically. The pattern relaxation time of 0.58 s, which is independent of the segment length, indicates a sorting and relaxation mechanism dominated by lipid diffusion rather than DNA self-diffusion.