Accessibility, Reactivity, and Selectivity of Side Chains within a Channel of de Novo Peptide Assembly
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- 作者：Antony J. Burton ; Franziska Thomas ; Christopher Agnew ; Kieran L. Hudson ; Stephen E. Halford ; R. Leo Brady ; Derek N. Woolfson
- 刊名：Journal of the American Chemical Society
- 出版年：2013
- 出版时间：August 28, 2013
- 年：2013
- 卷：135
- 期：34
- 页码：12524-12527
- 全文大小：293K
- 年卷期：v.135,no.34(August 28, 2013)
- ISSN：1520-5126
文摘
Ab initio design of enzymes requires precise and predictable positioning of reactive functional groups within accessible and controlled environments of de novo protein scaffolds. Here we show that multiple thiol moieties can be placed within a central channel, with approximate dimensions 6 脳 42 脜, of a de novo, six-helix peptide assembly (CC-Hex). Layers of six cysteine residues are introduced at two different sites 6 (the 鈥淟24C鈥?mutant) and 17 脜 (L17C) from the C-terminal opening of the channel. X-ray crystal structures confirm the mutant structures as hexamers with internal free thiol, rather than disulfide-linked cysteine residues. Both mutants are hexa-alkylated upon addition of iodoacetamide, demonstrating accessibility and full reactivity of the thiol groups. Comparison of the alkylation and unfolding rates of the hexamers indicates that access is directly through the channel and not via dissociation and unfolding of the assembly. Moreover, neither mutant reacts with iodoacetic acid, demonstrating selectivity of the largely hydrophobic channel. These studies show that it is possible to engineer reactive side chains with both precision and control into a de novo scaffold to produce protein-like structures with chemoselective reactivity.